Extraction conditions of polyphenol oxidase from banana peel
نویسندگان
چکیده
منابع مشابه
Banana Peel Oxalate Oxidase-detection, Purification, Characterization and Physiological Role
Objective: Enzymes like oxalate oxidase (EC 1.2.3.4) and superoxide dismutase (EC 1.15.1.1) from germin family are known to generate active oxygen species. In the mammalian system, excess accumulations of oxalate causes kidney stones. Oxalate oxidase, an H2O2-generating enzyme, used for detection of oxalate. The aim of the present work is to screen out the activity of enzymes from all three sta...
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The electrochemistry of banana tissues on a carbon paste electrode modified with multi-walled carbon nanotubes (MWCNTs) is presented. Cyclic voltammetry is applied to investigate the direct electrochemistry of banana tissues i.e. a source of polyphenol oxidase (PPO). A redox couple with an anodic and counterpart cathodic peak is obtained. The influence of various parameters such as pH,...
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Pectin is a complex polysaccharide and natural food additive extracted from citrus peel and apple pomace in the form of brownish powder. It is widely used as a jelling and thickening agent and the food industry especially in jams and jellies. It is also recommended for use as fat replacer and also lowers blood cholesterol by increasing viscosity in the intestinal tract thus lowering cholesterol...
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We produced a-amylase from banana peel by Bacillus subtilis through solid state fermentation (SSF). Addition of yeast extract, corn steep liquor, sodium dodesyl sulphate (SDS) and Tween-80 enhanced the a-amylase production under preoptimized fermentation conditions. Maximum enzyme yield (10.25 U/mL) was observed at 35°C after 24 hours of SSF in growth medium containing 0.1% yeast extract, 1% co...
متن کاملPartial Purification and Characterisation of Polyphenol Oxidase from Tomatoes (Solanum Lycopersicum)
Polyphenol oxidase (PPO) from tomatoes was extracted and partially purified through (NH4)2SO4 precipitation, dialysis and ion exchange chromatography. The activity of polyphenol oxidase was investigated in solanum lycopersicum. Spectrophotometric method was used to assay the enzyme activity and the kinetic constants - maximum enzyme velocity (Vmax) and Michealis - Menten constant (Km). Of the ...
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ژورنال
عنوان ژورنال: Baghdad Science Journal
سال: 2016
ISSN: 2411-7986,2078-8665
DOI: 10.21123/bsj.13.3.469-474